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Fig. 4. Interaction of atswc6 with other flowering-time mutants.
Comparison of Arabidopsis flowering phenotype in (A)
ld-1 and ld-1 atswc6,(B) fca-9 and fca-9
atswc6, (C) flc-3 and flc-3 atswc6, (D)
ft-1 and ft-1 atswc6. Photographs were taken after 40 days
in A and B, 20 days in C and 35 days in D. All plants were grown in long-day
conditions. (E) Northern blot analysis of FLC and
SOC1 in single and double mutants. The transcript level of
FLC was reduced by approximately half in double mutants (fca-9
atswc6, ld-1 atswc6) as compared with each single mutant. (F)
RT-PCR analysis of FLC and SOC1 in ft-1 and
ft-1 atswc6. TUB was used as an internal control. In E and F, RNA was
extracted from 10-day-old plants grown in long day conditions.
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