|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Knockdown of H2AZ causes a similar phenotype to atswc6.
(A) The RNAi construct targeting all three Arabidopsis H2AZ
genes - HTA8, HTA9 and HTA11 (AtH2AZs). The
tandemly fused HTA8, HTA9 and HTA11 cDNAs were arranged as
inverted repeats which were separated by a spliceable intron. The construct
was inserted between the CaMV 35S promoter and the OCS terminator. (B)
FRI and AtH2AZs RNAi transgenic plants grown for 45 days
under long-day conditions. (C) Distribution of flowering time in
AtH2AZs RNAi T1 transformants. Plants were grown in long-day
conditions. The number of plants in each category is given above each bar.
(D) Expression of HTA8, HTA9, HTA11 and FLC in three
AtH2AZs RNAi lines that flowered with 35, 30 and 30 leaves.
Homozygous lines were selected from the T2 generation of the three transgenic
plants and RNA was extracted from 10-day-old plants grown in long-day
conditions. All three AtH2AZs RNAi lines showed reduced expression of
HTA8, HTA9 and HTA11. The transcript level of FLC
was also reduced. (E) The relative levels of HTA8, HTA9 and
HTA11 transcripts in the three AtH2AZs RNAi lines as
compared with FRI wild type. (F) FRI, atswc6 FRI,
amiR-HTA8, amiR-HTA9, amiR-HTA11 and amiR-HTA9&11 T1 plants
grown for 40 days after germination in long-day conditions. (G) RT-PCR
analysis of expression of HTA8, HTA9, HTA11 and FLC in
FRI, atswc6 FRI, amiR-HTA8, amiR-HTA9, amiR-HTA11 and
amiR-HTA9&11 T1 plants. RNA was extracted from leaves of T1
plants. TUB was used as an internal control.
|
