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Fig. 5. Dynein is required for the efficient transport of grk RNA into
the oocyte and for its localization. (A-D) Wild type (A,B; wt) and
dhc mutant (C,D; dhc) stage-8 egg chambers injected with
grk RNA (pink) in the nurse cells. Notice the decrease in
grk RNA transport and localization in the oocyte in dhc
mutants (compare B' with D') (also see Movie 5 in the
supplementary material). (A-D) Grey is brightfield illumination showing the
outline of the nuclei and oocyte. Arrowheads indicate the position of the
oocyte nucleus.
(A',B',C',D') Injected
grk RNA only. (E-L) grk (E-H) and osk (I-L)
mRNA in situ hybridization in wild-type (E,F and I,J; wt) and dhc
mutant (G,H and K,L; dhc) egg chambers. (E,G,I,K) Stage 3-4 egg
chambers; (F,H) stage 6 egg chambers; (J,L) stage 11A egg chambers. Notice the
reduction in grk and osk mRNA signal and localization in the
oocyte of dhc mutants (arrowheads; compare E' with G' and
F' with H' for grk mRNA, and I' with K' for
osk mRNA). (L) In late-stage egg chambers, cytoplasmic foci of
osk mRNA on the periphery of dhc mutant egg chambers is
observed (arrows). (E-L) Cyan is DNA; red is mRNA; black and white panels show
mRNA only. In all panels, anterior is to the left and dorsal is to the
top.
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