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Fig. 5. Ureter differentiation occurs in zones of the UB surrounded by
Bmp4-expressing mesenchyme. (A) Sections of E14.5
Bmp4+/lacz embryos show abundant ß-gal staining,
indicating that Bmp4 expression persists in the mesenchyme (arrow)
surrounding the distal UB (dUB). ß-gal activity can also be detected in
glomerular cells (g). (B,C) Immunofluorescent analysis of E15.5
frozen sections demonstrates that this zone of the UB network (red, E-cadherin
staining) differentiates into the ureter, as determined by the presence of an
SMA-positive connective tissue coat (B, green) and upregulated uroplakin
expression (C, green). SMA-positive cells (B) are also detected around renal
arteries (ra). At E12.5 (D), the E-cadherin-positive UB network lacks
both UP-positive (D) and SMA-positive cells (data not shown). However, after
E12.5 rudiments were cultured for 4 days (E,F) the distal-most
domain of the E-cadherin-labeled UB (red) acquires a thick, SMA-positive
connective tissue coat (E, green) and exhibits upregulated expression of
uroplakins (F, green). (G) In situ hybridization detection of
Bmp4 in cultured rudiments indicates that ureter morphogenesis occurs
only in domains of the UB bounded by Bmp4-expressing mesenchyme.
Bmp4 mRNA can also be detected in glomerular podocytes (g).
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