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Figure 1


Fig. 1. Disruption of morphogenesis and maintenance of patterning in lulu mutants. Expression patterns at E8.5 assayed by immunofluorescence (A,B) or in situ hybridization (C-H). (A,B) 3D reconstructions from confocal z-stacks of wild-type (WT; A) and lulu (B) embryos stained with anti-Sox2 antibodies. (C,D) Krox20 is expressed in rhombomeres 3 and 5 of the wild-type hindbrain (C), and in the lulu neural plate (D, arrow). (E,F) Brachyury (T) expression in the notochord is discontinuous in lulu (F, arrows). (G,H) Meox1 expression in the paraxial mesoderm is unsegmented in lulu (H). All views are dorsal, except F, which is ventral. Anterior is up in all panels. Scale bars: 200 µm in A-D; 150 µm in E,F,H; 120 µm in G.





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