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Fig. 4. Defective EMT in the lulu primitive streak. E7.5
(A,B) and E7.75 (C-L) transverse sections of wild-type
(WT; A,C,E,G,I,K) and lulu (B,D,F,H,J,L) embryos. (A,B) In wild-type
and lulu embryos, E-cadherin is expressed in the epiblast but is
downregulated in the mesodermal wings (arrowheads). (C-H) Phalloidin labels
F-actin. Arrows in C,D mark the primitive streak; boxes are magnified in E,F;
bars mark the regions magnified in G,H. Mesodermal wings are thicker in wild
type than in lulu mutants (bars; E,F). F-actin is evenly distributed
around delaminating cells in the wild-type streak (arrowheads, G), whereas
cells in the lulu streak show spots of bright punctate F-actin
staining (arrowheads, H). (I,J) Anti-laminin staining marks the epiblast basal
lamina and highlights the increased width of the lulu streak compared
with wild type (brackets). The epiblast near the streak is also thicker in
lulu mutants (double-headed arrows). (K,L) Crumbs proteins are
apically concentrated in the wild-type (K) and lulu (L) epiblast but
are absent in involuting mesoderm. Anterior is to the left in A-D; apical is
up in E,F and to the left in G-L. Scale bars: 50 µm in A-D; 10 µm in
E-L.
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