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Fig. 7. Cytoskeletal alterations in the lulu neural plate. (A-H)
Transverse sections of E8.5 wild-type (WT; A,C,E,G) and lulu
(B,D,F,H) anterior neural plates; boxes in A,B show the equivalent positions
of the high-magnification images in C,E,G and D,F,H, respectively. (A-D)
Phalloidin reveals F-actin localization and tissue shape; ectopic
concentrations of F-actin appear away from the apical surface of the
lulu neuroepithelium (arrowheads, D). (E,F) Myosin IIB is
concentrated at ectopic sites in the lulu neural plate (arrowheads,
F). (G,H) Anti-phospho-ERM antibody recognizes activated (actin-binding) ERM
proteins; lulu neural plates show ectopic phospho-ERM staining away
from the apical surface. Scale bars: 25 µm in A,B; 5 µm in C-F; 10 µm
in G,H.
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