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Figure 1


Fig. 1. FP4mito alters Ena localization and phenocopies ena loss-of-function. (A) FP4mito. (B-C'') S2 cells, antigens indicated. (B) In wild-type cells (asterisk), Ena is diffusely cytoplasmic and enriched at leading edge (arrowhead) and cell contacts (white arrow). In FP4mito-expressing cells, GFP-FP4mito colocalizes with MitoTracker and Ena (yellow arrow). (C) FP4mito does not recruit WASP. (D-H) Embryonic CNS, BP102 antibody. (D) Control. (E,G) FP4mito x e22c-Gal4. (F) ena23/enaGC1. (H) ena210/enaGC1. Longitudinal (arrowhead) and commissural axons (arrow). (I) ISNb motor axon projections; wild type (left), ena mutants (right). (J-N) Projections of ISNb (arrows, anti-Fasciclin II) in embryos expressing indicated constructs (elav-Gal4). Scale bars: 10 µm.





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