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Fig. 3. Bmp signaling is not essential for the maintenance of specified liver
progenitors. Embryos obtained from outcrossing a hemizygous
Tg(hsp70l:dnBmpr-GFP) zebrafish were heat shocked at 22
(A-C,G-I,M-O) or 26 (D-F,J-L,P-R)
hpf, harvested at 33 (A-C,G-I), 36 (D-F,J-L) or 38-40 (M-R) hpf, and examined
for hhex (A-F), prox1 (G-L) and cp (M-R)
expression. The percentage of hemizygous embryos exhibiting a similar
expression pattern is indicated in the lower left corner (n=8-10).
When embryos were heat shocked at 22 hpf, both hhex and
prox1 expression in the liver region (arrows) were greatly reduced in
the hemizygous embryos, whereas their expression in other regions (arrowheads)
appeared unaffected (B,C,H,I). By contrast, when they were heat shocked at 26
hpf, both hhex and prox1 were clearly expressed in the liver
region in all the hemizygous embryos (E,F,K,L, arrows). However, hhex
and prox1 expression in the liver region (arrows) was somewhat
reduced compared with wild-type siblings, whereas hhex expression in
the pancreatic islet and prox1 expression in the interrenal
primordium (arrowheads) appeared unaffected (D-F,J-L). Hepatocyte
differentiation, assessed by cp expression, barely occurred in the
hemizygous embryos heat shocked at 22 hpf (N,O, arrows), and was clearly
reduced in those heat shocked at 26 hpf (Q,R, arrows). All images, except
insets, are dorsal views, anterior left. Insets are side views, anterior
left.
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