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Figure 5


Fig. 5. Gata4 and Gata6 are required for the expansion and differentiation of liver progenitors. Wild-type zebrafish embryos were injected with 10 ng gata4 MO and/or 2.5 ng gata6 MO, harvested at 37-38 hpf and examined for hhex (A-E), prox1 (F-J) and cp (K-N) expression. Injections of gata4 or gata6 MO weakly reduced hhex and prox1 expression in the liver region (B,C,G,H); injections of both MOs together strongly reduced their expression (D,E,I,J), but did not completely abolish it. Injections of gata4, gata6 or both MOs severely affected hepatocyte differentiation, as shown by cp expression (L-N). All images are dorsal views, anterior left (n=13-20).





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