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Fig. 7. Fgf signaling is not essential for the maintenance of specified liver
progenitors. Embryos obtained from outcrossing a hemizygous
Tg(hsp70l:dnfgfr1-EGFP) zebrafish were heat shocked at 22
(A-C,G,H,L-N) or 26
(D-F,I-K,O-Q) hpf, harvested at 35-36 (A-K) or 38-40
(L-Q) hpf and examined for hhex (A-F), prox1 (G-K), and
cp (L-Q) expression. The percentage of the hemizygous embryos
exhibiting a similar expression pattern is indicated in the lower left corner
(n=8-10). When embryos were heat shocked at 22 hpf, hhex
expression in the liver region (arrows) was greatly reduced (B) or almost
absent (C) in the hemizygous embryos, whereas its expression in the pancreatic
islet (arrowheads) appeared unaffected (B,C). prox1 expression in the
liver (black arrows) and retina (white arrows) was also greatly reduced in the
hemizygous embryos (H), whereas its expression in the interrenal primordium
appeared unaffected (H, arrowheads). By contrast, when embryos were heat
shocked at 26 hpf, both hhex and prox1 were clearly
expressed in the liver region in all the hemizygous embryos (E,F,J,K, arrows).
However, hhex and prox1 expression in the liver region was
reduced compared with wild-type siblings, whereas hhex expression in
the pancreatic islet and prox1 expression in the interrenal
primordium appeared unaffected (E,F,J,K, arrowheads). Hepatocyte
differentiation, assessed by cp expression, was severely defective in
the hemizygous embryos heat shocked at 22 hpf (M,N, arrows) and weakly reduced
in those heat shocked at 26 hpf (P,Q, arrows). All images, except insets, are
dorsal views, anterior left. Insets are side views, anterior left.
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