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Fig. 8. Drosophila Nemo phosphorylation promotes the nuclear export of
Mad. COS-7 cells were transfected with the constructs indicated and
stained for the localization of Mad (green, upper panel of each pair) and with
DAPI to indicate nuclei (blue, lower panel of each pair). MadS25A is primarily
nuclear (A), whereas MadS25D is heavily enriched in the cytoplasm
(B). Percentages indicate the number of cells displaying a primarily
nuclear localization. (C,D) The localization of Mad is
influenced by Tkv receptor activation. (E,F) Co-transfection of
Nemo inhibits the Tkv-induced nuclear accumulation in the absence of
leptomycin B (LMB) (E), but does not block nuclear retention in the presence
of LMB (F).
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