DEV02852 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Clone 1. A single dorsal cell divides into two cells (day 6) and then one disappears by day 10.

• Supplemental Figure S2 -

  Fig. S2. Clone 2. Cell starts in a lateral position and then moves dorsally by day 5. By day 7 the cell has divided and divides again by day 10. By day 12 the cells have spread along the dorsal side.

• Supplemental Figure S3 -

  Fig. S3. Clone 3. Dorsal cell has divided into two cells by day 13.

• Supplemental Figure S4 -

  Fig. S4. Clone 4. Dorsal cell divides by day 8 and becomes a four-cell clone by day 16. Sectioning indicates that cells are in the PAX7⁺ zone. Red, PAX7 immunohistochemistry; blue, Hoechst staining for nuclei.

• Supplemental Figure S5 -

  Fig. S5. Clone 5. Spinal cord contains two separate cells in the dorsal domain. The arrowhead follows the anterior cell whereas the posterior cell disappears. By day 6 the anterior cell has divided. By day 10 it has divided further to form a clone of three cells. Some of the cells have disappeared by day 26.

• Supplemental Figure S6 -

  Fig. S6. Clone 6. Single cell in dorsal domain divides into two cells and spreads along the dorsal domain. One cell has disappeared by day 10.

• Supplemental Figure S7 -

  Fig. S7. Clones 7 and 8. Three cells are initially marked along the dorsal domain, two dimmer cells and one brighter cell. In several samples the brighter cells often disappeared over time and thus were not tracked. Arrows indicate the two dimmer, anterior cells that divided to produce clones in the dorsal domain. The anterior-most cell is marked with a red arrow and the more-posterior cell is marked with a black arrow. By day 9 the anterior cell (red arrow) has divided into four cells along the dorsal domain. The GFP signal becomes dimmer but the cells are still visible at day 31. The more-posterior cell (black arrow) produces a three-cell clone by day 9, which divides to give a five-cell clone by day 19 and then a seven-cell clone by day 29.

• Supplemental Figure S8 -

  Fig. S8. Clone 10. A dorsal cell divides by day 9 and the daughters move apart so that one cell lies dorsal and the other lies lateral.

• Supplemental Figure S9 -

  Fig. S9. Clone 11. A dorsal cell at day 3 divides by day 5 to generate two daughters that have a dorsal location, but by day 7 the cells have spread apart with one daughter positioned laterally. By day 14 the clone is now three cells that are repositioned by day 24 to give dorsally and laterally positioned cells.

• Supplemental Figure S10 -

  Fig. S10. Clone 12. A cell that starts in a lateral position divides by day 5 to give two lateral daughters. One daughter is no longer visible by day 11.

• Supplemental Figure S11 -

  Fig. S11. Clone 13. A dorso-lateral cell at day 3 has divided into two daughters by day 5 that move more laterally by day 7.

• Supplemental Figure S12 -

  Fig. S12. Clone 14. A dorso-lateral cell remains as a single cell until day 11. By day 13 it has generated four daughters that are all dorso-laterally positioned.

• Supplemental Figure S13 -

  Fig. S13. Clone 15. A single lateral cell divides at day 11 to produce two lateral daughters that spread along the A/P axis.

• Supplemental Figure S14 -

  Fig. S14. Clone 16. A single lateral cell divides by day 7 and further divides by day 10 to produce a row of lateral cells.

• Supplemental Figure S15 -

  Fig. S15. Clone 17. A lateral cell divides by day 7. These daughters separate from each other by day 10 and divide by day 14 to produce a four-cell clone. These cells further divide by day 16 to give a seven-cell clone. Eight cells are visible by day 18 and 10 cells by day 24. Some of the anteriorly positioned cells have moved into a ventral position.

• Supplemental Figure S16 -

  Fig. S16. Clone 18. A lateral cell (black arrowhead) divides by day 5 giving two dorso-lateral daughters. By day 10 the daughters (black arrowhead) are dorsally positioned and have separated along the A/P axis.

• Supplemental Figure S17 -

  Fig. S17. Clone 19. A single ventral cell divides to give a ventral and lateral daughter by day 7.

• Supplemental Figure S18 -

  Fig. S18. Clone 20. A ventrally positioned cell gives two daughters by day 5 that lie laterally. The cells divide into a four-cell clone by day 7 and a six-cell clone by day 11. Some of the cells have translocated laterally by day 13, and the clone consists of seven cells. The first image of the cell on day 3 was taken with a 40× objective whereas the rest were imaged using a 25× objective.

• Supplemental Figure S19 -

  Fig. S19. Clone 22. Tracing of a cytoplasmic GFP-labeled cell. The marked cell starts in a dorsal location at day 6. A2 is a higher magnification image of A1. A3 is the fluorescence image only. By day 9 the cell has divided and the daughters are now in a lateral position (B1, white arrowhead). B2 represents a higher magnification image and B3 is a fluorescence-only image. By day 11 the cells have further expanded and distributed into lateral and dorsal domains. C2 represents a higher magnification image and C3 is a fluorescence-only image. By day 14 the cells span ventral, lateral and dorsal domains. D2 represents a higher magnification image and D3 is a fluorescence-only image. The cell group is positioned relatively close to the terminal vesicle.