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Figure 5


Fig. 5. The ventrally derived clone generated PAX6- and PAX6+ daughter cells. The tail shown in Fig. 4 was fixed at day 23 and sectioned transversally. Sections containing nuclear GFP+ cells (A,D) were double immunostained for PAX6 (B,E; rhodamine fluorescence) and PAX7 (C,F; Cy5 fluorescence, shown in red, arrowheads). (A,B) The ventrally located nuclear-eGFP-expressing cell is PAX6- (arrows). (D,E) The GFP+ cells in the lateral domain are PAX6+ (arrows with asterisk). Arrows, eGFP+ and PAX6- cells; arrows with asterisk, eGFP+ and PAX6+ cells; arrowheads, PAX7-expressing cells.





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