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Figure 5


Fig. 5. Ectopic activation of Shh signaling in the dorsal region of the ShhN/- telencephalon. (A,B) E14.5 Ptch1-lacZ (A) and ShhN/-;Ptch1-lacZ mouse coronal sections stained with X-Gal to highlight sites of Shh signaling in the telencephalon. In control embryos, lacZ expresssion is restricted to the basal ganglions, with weaker lacZ expression in the LGE domain (A, arrow). In ShhN/- embryos, weak lacZ expression is additionally detected at many sites in the dorsal telencephalon (B, arrowheads), consistent with ectopic LGE marker expression. (C) Immunoblotting of whole-brain protein extracts followed by incubation with a Gli3-specific antibody recognizing full-length (Gli3-190) and repressor forms (Gli3R) of Gli3. The Gli3R/Gli3-190 ratio is reduced in the E15.5 ShhN/- brain. (D) Bar chart showing Gli3R/Gli3-190 ratio.





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