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Fig. 7. Expression of Shh RNA, protein and target genes in Shh^N/- neural tube. (A-H) Comparison of Shh RNA and protein expression in control and Shh^N/- mouse forebrains. At the nine-somite stage, Shh expression can be detected in the forebrain neuroepithelium (A,E, arrowheads) and the underlying dorsal foregut endoderm (arrows). Note that Shh RNA expression is selectively lost in Shh^N/- telencephalon (E,F, arrowheads). Similarly, Shh protein is also undetectable in the ventral Shh^N/- telencephalon (H, arrowhead in G), and protein expression in the dorsal foregut is significantly reduced at the eight-somite stage (G, arrow). The dotted line (B,F) encloses optic vesicles. (I,J,M,N) Nkx2.1 and Ptch1 expression in control and Shh^N/- forebrain at E9.5. The dotted line encloses optic vesicles. (K,L,O,P) Comparison of expression of Shh RNA and protein in control and Shh^N/- spinal cord at E10.5. Shh protein is detected in both the basal and apical surfaces of the floorplate as well as in ependymal cells that line the central canal of the neural tube (L, arrows). However, in Shh^N/- embryos, Shh protein resides exclusively at the apical compartment of floorplate cells (arrowheads in P) and does not accumulate at the ependymal cell surface. n, notochord.

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