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Fig. 8. ShhN rescues patterning defects in the spinal cord but not in the telencephalon of Disp1^-/- mouse embryos. (A-E) The Disp1^-/-;Shh^N/+ mutant telencephalon shows characteristics similar to those of the Shh^N/- telencephalon, including absence of Shh (A) and Nkx2.1 (E) expression, enlarged forebrain ventricles (B, the region enclosed by the dotted line), reduced eye (B, arrow), ventrally extended Pax6 expression (C) and presence of Mash 1 expression (D). Arrows in C and D mark the presumptive boundary between LGE and cortex. (F) Robust Shh protein signal is visualized in notochord and floorplate. Note the absence of Shh protein staining in ependymal cells. (G-J) Dorsoventral patterning is established in Disp1^-/-;Shh^N/+ mutant spinal cord, as demonstrated by defined expression domains of Pax7 and Nkx6.1 (G). Note the presence of V3 interneuron as shown by Nkx2.2 staining (I) and the expanded pool of floorplate cells (H).

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