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Figure 4


Fig. 4. HOW(L) represses the levels of DPP-GFP in a 3'UTR-dependent manner. (A-L) Wing imaginal discs labeled with GFP (A,D,G,J) or with anti-HOW (red; B,E,H,K) were dissected from larvae carrying the vestigial-gal4 driver and UAS-HOW(L) (D-F,J-L), and either a dpp-GFP fusion construct that contains the dpp3'UTR (A-F) or one lacking the dpp 3'UTR (G-L). The corresponding merged panels are shown (C,F,I,L). Arrows show reduced GFP expression in the presence of HOW(L) and dpp3'UTR, and normal GFP expression in the absence of the 3'UTR of dpp. Note a significant reduction of DPP-GFP in the presence of HOW(L) only when the dpp-GFP construct contains the endogenous dpp 3'UTR (D).





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