DEV02850 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Increased cell-scatter following HH blockade in the midbrain. Unilateral HH blockade results in the spread of PHOX2A+ precursors and differentiated neurons in a cross-section through an E6 midbrain. Broken line delineates the ventricular-mantle layer boundary.

• Supplemental Figure S2
• Supplemental Figure S3 -

  Figs S2 and S3. Cell death following HH blockade in the midbrain. TUNEL labeling at E5 demonstrating a comparable increase in cell death on the electroporated (right) side after unilateral EGFP (S2) and Ptc1^Δloop2 (S3) electroporations.

• Supplemental Figure S4 -

  Fig. S4. Reduced cell proliferation following SHH overexpression in the midbrain. Unilateral misexpression of SHH results in reduced cyclin D1 expression (*) in tectum.

• Supplemental Figure S5 -

  Fig. S5. The lateral periphery of the ventral midbrain is specified by H&H 13. Cross-section through the brain shown in Fig. 4G and H, demonstrating that HH blockade after H&H 13 does not affect the specification of lateral (EVX1+) cell fates.

• Supplemental Figure S6 -

  Fig. S6. Cell mixing following HH blockade in the midbrain. HH blockade results in the mixing of OTX2+ (blue, arrowhead) and hindbrain cells (arrowhead). Although GBX2 (blue) expression is present in lateral hindbrain, it is absent from the hindbrain ventral midline where cells are electroporated and ectopic OTX2 is seen.