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Figure 4


Fig. 4. Wnt and FGF signalling inhibit neurogenesis via different mechanisms. (A-C') Neural tube (NT) explants in control media do not express Wnt8c at the time of excision or 24 hours later (A) but do express Wnt1 (B) and Wnt3a (C). FGF4 does not reactivate Wnt8c (A'), reduces strongly Wnt1 (B') and mildly Wnt3a (C'). (D-E') NT explants in control media with ethanol (D,E), CKI-7 (D') or FGF4 and CKI-7 (E'). Inhibiting Wnt signalling with CKI-7 does not alter NeuroM expression in NT (within 24 hours) and FGF4 in the presence of CKI-7 is still able to inhibit NeuroM (24 hours, compare E with E' and see Fig. 3G,G'). (F,F') RARß is expressed in the transition zone and the underlying rostral presomitic mesoderm at HH10 (F': section of F indicated by a white line). (G,G') NT explants in control media (G), with FGF4 (G'). pNT explants combined with control cells (H) or Wnt8c cells (H'). PSM explants combined with control cells (I) or Wnt8c cells (I'). RARß is dramatically inhibited by FGF4 in neural tube explants, but is not consistently affected by Wnt8c in pNT. RARß appears enhanced in underlying PSM by Wnt8c in 24 hours of culture. Scale bars: 50 µm in A for A-C',H-I', in D for D-E',G-G' and in F'; 100 µm in F.





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