DEV002345 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. RGC growth cones make extended pauses after depleting Xena/XVASP function. In vivo time-lapse sequence of control GAP-GFP (A) and FP4-Mito-RFP+GAP-GFP (B) expressing axons at the tectum border. Pictures illustrate growth cones at 30-minute intervals. White arrows point to filopodia and dashed lines indicate growth cone progression between pictures. Scale bars: 22 μm in A,B.

• Movie 1 -

  Movie 1. In vivo time-lapse sequence. 5 hours in vivo time-lapse sequence at 5-minute intervals of control GAP-GFP-expressing growth cones growing along the optic tract.

• Movie 2 -

  Movie 2. In vivo time-lapse sequence. 4 hours 30 minutes in vivo time-lapse sequence at 15-minute intervals of FP4-Mito-RFP+GAP-GFP-expressing growth cones growing along the optic tract.

• Movie 3 -

  Movie 3. In vivo time-lapse sequences. 4 hours in vivo time-lapse sequence at 5-minute intervals of control GAP-GFP axon terminal arborizations within the tectum.

• Movie 4 -

  Movie 4. In vivo time-lapse sequences. 5 hours in vivo time-lapse sequence at 10 minute intervals of terminal arborizations of FP4-Mito-RFP+GAP-GFP-expressing axon within the tectum.