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Fig. 4. Function of the Xena/XVASP proteins is not required for axon pathfinding
in the optic tract. (A-E) Lateral views of stage 39 whole-mount
brains illustrating the axons of control GAP-GFP-expressing (A,B) and
FP4-Mito-RFP+GAP-GFP-expressing (C-E) RGC neurons. The solid line approximates
the location of the rostral border of the tectum. (A,B) Control axons labeled
with GAP-GFP grew in the medial and dorsal optic tracts (MOT and DOT,
respectively), turned caudally in the mid-diencephalon (Di) and penetrated
into the tectum (Tect), where they arborized. (C) Axons of neurons expressing
FP4-Mito navigated correctly. However, only very few retinal axons with
impaired function of the Xena/XVASP proteins were detected in the optic tract
or in the tectum. (D,E) We confirmed that the GAP-GFP axons in D expressed the
Mito construct by detecting the presence of FP4-Mito-RFP on the mitochondria
(E; arrows). CH, optic chiasm; Di; mid-diencephalon; DOT/MOT/VOT,
dorsal/medial/ventral optic tract, respectively; Tect, tectum; Tel,
telencephalon. Scale bar: 84 µm in A; 42 µm in A for B,C; 30 µm in A
for D-E.
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