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Fig. 2. Synchondrosis growth plate organization and chondrocyte proliferation
are deranged in Kif3a-deficient cranial bases. (A-D)
Parasagittal sections of a P7 control spheno-occipital synchondrosis. Notice
the presence of resting (rz), proliferative (pr),
pre-hypertrophic (phz) and hypertrophic (hz) growth plate
zones and primary bone spongiosa (arrowheads in D). Areas in colored frames in
A are shown at higher magnification in B-D. (E-G) Presence and location
of proliferating chondrocytes in control P0 (E), P7 (F) and P15 (G)
spheno-occipital synchondroses as revealed by histone 4C gene expression by in
situ hybridization. The hybridization signal was given an artificial color for
illustration purpose. Notice the presence of two well-defined proliferative
zones (indicated by arrowheads) flanking a central resting zone. (H)
Pthrp gene expression in a control P0 synchondrosis, which
characterizes the resting and proliferative zones. (I-L) Parasagittal
sections of a P7 Kif3a-deficient spheno-occipital synchondrosis
showing that the growth plate zone structure is totally abnormal (J,K) and
that there is a near absence of primary spongiosa (L). (M-O) Near
absence of histone 4C-expressing proliferating chondrocytes in
Kif3a-deficient synchondroses. (P) Pthrp gene
expression in a Kif3a-deficient synchondrosis. Scale bars: 300 µm
in I for A,I; 40 µm in J for B,C,J,K; 80 µm in L for D,L; and 150 µm
in M for E-H and M-P. P, postnatal day;
rz/pr/phz/hz,
resting/proliferative/pre-hypertrophic/hypertrophic growth plate zones.
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