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Figure 7


Fig. 7. Topography of hedgehog signaling is altered in Kif3a-deficient synchondroses. Serial sections of P0 control (A-L) and mutant (M-X) intrasphenoidal (is) and spheno-occipital (so) synchondroses were processed for expression analysis of the indicated genes. (C,D,I,J) Notice that, in controls, Patched 1 (C,I) and Gli1 (D,J) were expressed in the proliferative zone (pz) and in the perichondrium flanking the pre-hypertrophic and hypertrophic zones (single arrowheads), but not in the perichondrium flanking the resting and proliferative zones (double arrowheads). (O,P,U,V) In mutants, however, Patched 1 (O,U) and Gli1 (P,V) are minimally expressed within the growth plates, but are expressed all along the perichondrial tissues (arrowheads). (F,L,R,X) Notice also that syndecan 3 gene expression is mainly restricted to the proliferative zone in controls (F,L), but is extremely low in mutants (R,X). Expression of Ihh and smoothened was similar in control (B,H,E,K) and mutant (N,T,Q,W) tissues at this stage. is, intrasphenoidal; pz, proliferative zone; so, spheno-occipital. Scale bar: 150 µm in A for A-X.





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