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Fig. 8. Ihh distribution is altered in Kif3a-deficient
synchondroses. (A-D) Serial sections of P0 control (A,B) and
Kif3a-deficient (C,D) spheno-occipital synchondroses were processed
for immunohistochemistry using Ab80 rabbit hedgehog antibodies. Sections were
reacted with secondary fluorescent antibodies and counterstained with the
nuclear dye DAPI (blue). Positive immunosignal is orange in color. (A,B) In
controls, Ihh is present in the pre-hypertrophic zone (phz) and in
adjacent proliferative chondrocytes and inner perichondrium (B, single
arrowhead), but is undetectable in the upper growth plate zones and flanking
perichondrium (B, double arrowhead). (C,D) However, in
Kif3a-deficient specimens, Ihh is present in a more extensive and
expansive gradient form throughout much of growth plate and all along
perichondrium (D, single and double arrowheads). (E,F) Notice
that the hedgehog antibodies produced no detectable signal with sections from
Ihh-null synchondroses, attesting to their specificity. (B,D,F)
Higher-magnification images of the boxed area in A,C and E, respectively.
rz/pr/phz, resting/proliferative/pre-hypertrophic
growth plate zones. Scale bar: 65 µm in F for B,D,F.
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