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Fig. 9. Conditional postnatal Ihh deficiency causes cranial base
abnormalities. (A,E) Skulls from P15 control (A) and
Ihh-deficient (E) mice were subjected to micro-computed tomography
(µCT) analysis; one orthogonal plane through the cranial base of each is
shown here. Notice the presence of well-defined intrasphenoidal (is)
and spheno-occipital (so) synchondroses in controls (A), and the
ill-defined synchondroses and reduced anteroposterior length in mutants (E).
(B-D,F-H) Sections from P7 and P15 control (B-D) and mutant
(F-H) so synchondroses processed for collagen X gene expression (B,F)
or for histological analysis (C,D,G,H). Notice that collagen X transcripts
(red) are restricted to hypertrophic zones in controls (B) but are widespread
throughout the mutant synchondrosis (F). Notice also the presence of a
well-formed intramembranous bone collar flanking the pre-hypertrophic and
hypertrophic zones in controls (C, arrowheads), which is undetectable in
mutants (G, arrowhead). (D,H) Additionally, in mutants, much of the
synchondrosis is replaced by endochondral bone by P15 (H). is,
intrasphenoidal; so, spheno-occipital. Scale bar: 2 mm in E for A,E;
150 µm in F for B,F; 75 µm in G for C,G; and 250 µm in H for D,H.
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