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Figure 1


Fig. 1. GDNF expression is restricted to the distal gut after colonization of the entire gut by ENS progenitors. (A) GDNF expression in gut at E15.5. The gut isolated from GDNFlacZ/+ mouse embryos was stained with X-gal histochemistry. Intense X-gal staining was observed in the cecum and colon. (B) Wholemount preparations of the small intestine (upper panels) and colon (lower panels) of E15.5 GFR{alpha}1GFP/+ embryos (obtained by crossing GFR{alpha}1flox/+ mice to ß-actin Cre mice: see Materials and methods). GFR{alpha}1-expression (as revealed by GFP) was confirmed in almost all PGP9.5+ neurons in the colon, whereas only a small neuronal population expressed GFR{alpha}1 in the small intestine. (C) Quantitative analysis of GFR{alpha}1-expressing neuronal population. Percentage of GFP+-PGP9.5+ neurons / total PGP9.5+ neurons is presented. Scale bar: 10 µm in B. Co, colon; SI, small intestine.





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