|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Spatiotemporally specific effects of
GFR
1 inactivation on ENS development in
mouse. (A) Confocal microscopic images of wholemount preparations
of midgut from E13.5 control and cKO embryos in which
GFR1 inactivation was induced at E11.5.
GFP+ cells were less dense in the cKO than control midgut.
(B) Wholemount PGP9.5 staining of small intestine from E18.5 control
and cKO embryos subjected to 4-OHT treatment at E13.5. Although total colon
aganglionosis was observed in cKO embryos (bottom right), the ENS structure in
the small intestine was well maintained (top right). (C) Wholemount
preparation of myenteric plexus of the colon from P14 mice subjected to
GFR1 inactivation at P5. No obvious abnormalities were found in enteric
neurons (PGP9.5+) or GFP+ cells of cKO myenteric plexus
(upper panels). Even multiple 4-OHT injection did not affect ENS structure
(lower panels). Recombination efficiency by single 4-OHT injection was
estimated as 50% for A and B, and 40% for C. S, sacrificed. Scale bars: 10
µm in A; 50 µm in B; 100 µm in C.
|
