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Fig. 1. Mutations in the dlkb1 gene disrupt spindle organization of
both NBs and GMCs. (A) Map of the Drosophila lkb1
(dlkb1) gene and its genomic region. P[dlkb1+]
designates the genomic fragment that rescues both the lethality and the
cytological defects associated with the dlkb1 mutation. Black boxes
correspond to protein-coding exons, and the arrows indicate the direction of
transcription. The positions of the stop codons causing the
dlkb1315 and dlkb17 mutations are
indicated by vertical lines. (B) Mitotic spindle morphology of NBs and
GMCs from wild-type (wt) and dlkb1 brains. Cells were stained for
tubulin (Tub, green) and DNA (blue). (C) Spindle morphology of
wild-type (wt), dlkb1, asl,and asl dlkb1 metaphases stained
for tubulin (green), DNA (blue) and Centrosomin (red). Note that the spindle
density in asl dlkb1 double mutants is substantially lower than in
asl single mutants. Scale bar (all panels): 5 µm.
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