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Fig. 3. Mutations in the Drosophila lkb1 gene disrupt unequal cytokinesis and reduce the average size of NB population within mutant brains. (A) Wild-type and dlkb1 metaphases stained for tubulin (Tub) DNA and Deadpan (Dpn). Note that the NBs are more intensely stained by the anti-Dpn antibody than GMCs. Scale bar: 5 µm. (B,C) Size distribution of metaphase (B) and ana-telophase (C) spindles in wild-type, dlkb1³¹⁵/Df(3R)urd, pins^P62/pins^P62 and asl²/asl² brains. Dpn-positive (NB) and Dpn-negative (GMC) spindles are depicted in red and green, respectively. Size (µm) classes in B: A, 4.7-6.9; B, 7.0-9.2; C, 9.3-11.5; D, 11.6-13.8; E, 13.9-16.1; F, 16.2-18.4; G, 18.5-20.7; H, 20.8-23.0; I, 23.1-31.7. Size (µm) classes in C: J, 6.7-11.2; K, 11.3-15.8; L, 15.9-20.4; M, 20.5-25.0; N, 25.1-29.6; O, over 29.7. The numbers above each column indicate the number of cells observed in each size class. (D) Criterion used for measuring the asymmetry index of NB divisions. The difference between the length of the long (a) and the short (b) spindle axis (a-b) was divided by the total length of the two axes (a+b). Scale bar: 5 µm. (E) Asymmetry indexes in wild-type, dlkb1³¹⁵/Df(3R)urd, pins^P62/pins^P62 and asl²/asl² NBs of different sizes. The bars correspond to the s.e.m. Size (µm) classes: P, 11.3-15.8; Q, 15.9-20.4; R, 20.5-25.0; S, 25.1-29.6; T, over 29.7.

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