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Figure 4


Fig. 4. The cardiogenic mesoderm is required for liver specification. (A) At the 32-cell stage, Xenopus embryos were injected with Gsk3ß RNA (500 pg) into the D4 posterior endoderm cell. At stage 18, endoderm explants were isolated from control and injected embryos, and cultured with or without their associated mesoderm (orange) until stage 35 when they were assayed by RT-PCR. Foregut explants from uninjected embryos were either left intact with the anterior endoderm and associated mesoderm (AEM), or the anterior endoderm (AE, green) was separated from the mesoderm (AM). Posterior endoderm and mesoderm (PEM), or posterior endoderm without its associated mesoderm (PE), was also isolated from control and Gsk3ß-injected embryos (PE+Gsk3ß). (B,C) Bar charts showing the normalized relative mRNA expression levels from RT-PCR of the liver marker for1, heart marker cardiac-troponin and the pan-endodermal marker endodermin. WE, whole embryo; AE+AM, endoderm and mesoderm separated and immediately recombined.





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