|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Cauterization of the CR does not change the dorsoventral level of rib
growth. (A-C) Transverse sections of a stage 14 P.
sinensis embryo fixed just after unilateral microcauterization. (A)
Enlargement of the experimental side of the embryo (B) shows necrosis of the
CR tissue (arrowhead). (C) Enlargement of the left side of the same embryo
showing the intact CR as the negative control. (D-G) In situ
hybridization of an embryo 1 day after cauterization with a probe for
CRABP1 (D,E) or for APCDD1 (F,G). The left panels represent
the operated sides, and the right panels represent the corresponding control
sides. In both cases, CR-specific gene expression is lost from the epidermis
of the cauterized CR. (H-J) Three days after the microcauterization of
the CR performed at stage 14. (H) A magnified view of the experimental side of
the embryo; the image on the right shows an enlargement of the intact CR. Note
that the intact CR comprises a thickened epidermis covering the accumulated
mesenchyme (J), whereas on the operated side, these characteristic features of
the CR have been lost (H). (I) Also note that the transversely viewed patterns
of the ribs (r) are identical on the control and operated sides. (K-N)
A whole-mount embryo 12 days after CR cauterization. Control (K) and operated
(L) sides. Note the arrested growth of the carapacial margin at the site of
cauterization (arrowhead). Dorsal (M) and lateral (N) views of the skeleton of
the same embryo stained with Alcian Blue. Two successive ribs approximate each
other distally (red arrowheads) at the site of CR cauterization (black
brackets). Compare with the normal fan-shaped pattern on the control side
(white brackets). Note that the ribs at the CR ablation do not grow ventrally
across the level of the ablated CR (black arrowhead in N). (O-R) A
whole-mount embryo 10 days after the cauterization of the apical ectodermal
ridge of the forelimb. Control (O,P) and operated (Q,R) sides. (P,R) Alcian
Blue-stained embryo. Note the severely disfigured autopod on the operated side
(Q,R). Scale bars: 50 µm for A,C,H,J; 100 µm for B,D-G; 500 µm for I;
1 mm for K-R.
|
