QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 3. Development of neurons, but not glia, in explants of fetal gut is Hand2-dependent. (A) Transcripts encoding Phox2b detected by reverse transcriptase (RT)-PCR in fetal mouse gut at E9.5 in wild-type (+/+), Hand2^+/- and Hand2^-/- mice. (B) Sox10+, Phox2b+ and Ret+ cells are detected at E9.5 in the outer mesenchyme of wild-type (H2+/+) and Hand2^-/- (H2-/-) gut. (C) p75^NTR+ cells are found in longitudinal sections cut through the foregut of wild-type and Hand2^-/- mice. (D) Hu+, PGP9.5+ and NSE+ neurons develop in cultures of wild-type gut explanted at E9.5. (E) B-FABP+ (red), as well as Hu+ (green), neurons develop in cultures of wild-type gut explanted at E9.5. B-FABP+ glia, but no Hu+ neurons, develop in cultures of Hand2^-/- gut explanted at E9.5. (F-H) Sox10 (F), Phox2b (G) and Ret (H) immunoreactivities are found in cultured explants of E9.5 gut from Hand2^-/- and wild-type mice. (I) p75^NTR+ precursors (red) are present in explants of E9.5 gut from wild-type and Hand2^-/- mice; however, Hu+ neurons (green) develop in vitro only in the explants of wild-type, and not Hand2^-/-, gut. (J) Co-culture of E9.5 Hand2^-/- gut with somites containing primordial dorsal root ganglia. Hu+ neurons (green) develop. (K) Differentiation of neurons occurs when Hand2^-/- explants are rescued by transfection with Hand2; neurons co-express Hu (green) and Hand2 (red) immunoreactivity. Ctrl, control. Scale bars: 50 µm in B-J; 10 µm in K.

Return to article