spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Silencing of Hand2 prevents the in vitro development of enteric neurons. (A) Crest-derived cells immunoselected from E13 mouse gut, cultured and transfected with siRNAHand2/GFP or siRNAscrambled/GFP. siRNAHand2/GFP interfered with the development of Hu+ neurons (pink). Notice the GFP-fluorescent axons (green) of the Hu+ neurons in cultures transfected with siRNAscrambled/GFP (A; left), which are absent in those transfected with siRNAHand2/GFP (A; right). (B) Quantitative results. Overall density both of Hu+ neurons and transfected cells developing as neurons (doubly labeled with GFP and Hu) in cultures transfected with siRNAHand2/GFP are lower than those seen in cultures transfected with siRNAscrambled/GFP. siRNAHand2/GFP does not differ from siRNAscrambled/GFP in transfection efficiency measured on non-neuronal cells. Scale bar: 50 µm.





Right arrow Return to article