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Figure 6


Fig. 6. Terminal differentiation of enteric neurons, but not glia, is abnormal in Wnt-1-CreH2{Delta} mice. (A) nNOS+ (red) and Dbh+ (green) neurons are found in the presumptive enteric nervous system (ENS) of E12 wild-type (Wt) mice but not in Wnt-1-CreH2{Delta} bowel. (B) Hu+ (red) neurons and B-FABP+ (green) glia are present in the stomachs of wild-type mice at E12. Wnt1-CreH2{Delta} stomachs contain B-FABP+ glia but almost no Hu+ neurons. (C) The densities of B-FABP+ cells in Wnt1-CreH2{Delta} and wild-type mice are similar. (D) The number of gut cells revealed by the TUNEL technique to be undergoing apoptosis is similar in Wnt1-CreH2{Delta} and wild-type mice. The dorsal root ganglion (DRG) and neural tube were studied as positive controls. DRG, dorsal root ganglion; g, gut; L, liver; ns, not significant. Scale bars: 50 µm.





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