QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 7. Loss of Fgf8b expression alters the orientation of the AP axis, but not the long axis, of the embryo with respect to the longitudinal axis of the uterine horn. (A) Schematic of orientation the of the embryo within the uterus and the relative position of the sectioning plane. (B) Schematic representation of the relationships between the axes of the embryo and uterus found in two groups (I and II) of embryos at E6.5 from intercrosses of Fgf8^+/b parents. (C,D) Immunohistochemical analysis of EGFP, which marks the anterior visceral endoderm (AVE; brown) on transverse sections of E6.5 embryos within the uterus. Insets show embryos at higher magnification. (E,F) In situ hybridization of T transcripts on cross sections of wild-type and Fgf8^b/b embryos at E7.5 within the uterus. (A-F) Red arrow represents the AP axis; black double-headed arrow marks the long uterine axis. Notice the abnormal bulge of cells (arrowhead) at the primitive steak of Fgf8^b/b embryos in F. (G) Distribution of the angles () between the long axes of the embryo and the uterus at E6.5 for groups I and II. Each dot represents one embryo. The average angles (horizontal blue and red bars) and standard deviations (vertical bars) are indicated. (H) Distribution of the angles () between the AP axes and the uterus at E7.5 for control (red) and Fgf8^b/b (green) embryos relating to the ratio of the AP:LR dimension. Green and red broken lines indicate the average angle for control and Fgf8^b/b embryos, respectively. A, anterior; D, distal; L, left; P, posterior; Pr, proximal; R, right.

Return to article