DEV02856 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Fine mapping of dSno¹⁷⁴. Genomic region in the vicinity of dSno. Schematic representation of a 14.5 kb region surrounding CG7233 (dSno, red bar) that is transcribed from the minus strand (FlyBase, 2005). Blue bar depicts CG7231. Green arrowhead indicates the P-element l(2)Sh1402 inserted 719 bp upstream of the dSno translational start. Deletions of dSno were generated by mobilizing this P element. PCR amplification was performed on the potential deletions obtained in the screen. Black dotted line depicts the genomic region missing in the original P-element (297{}323 − a retroposon in the region). Several deletion lines were generated (data not shown). The dark-red dotted line represents the deletion dSno¹⁷⁴, which was used in this study. The mapping of this deletion was based on three different amplicons with different sizes. Primer combinations were as follows: F1R1, 13,338 wild-type (bp) and 4300 dSno¹⁷⁴ (bp); F2R2, 1360 bp and no PCR product; and F2R3, 563 bp and no PCR product. The PCR product obtained using the primer F1R1 on genomic DNA from dSno¹⁷⁴ was cloned and sequenced. The product had a deletion of 2.2 kb that removes the entire CG7233.

• Supplemental Figure S2 -

  Fig. S2. dpp RNA expression and pMAD distribution in egg chambers mutant for dSno. Lateral views of the posterior half of stage 10B egg chambers. (A,B) In situ hybridization for dpp mRNA. (C-E). Anti-pMAD antibody stainings. (A,C) Wild type. (B,D) dSno¹⁷⁴/dSno¹⁷⁴. (E) brk ^M68/+; dSno¹⁷⁴/dSno¹⁷⁴.