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Fig. 10. DiI labelling of commissural neurons in P10 CxCdk5KO and
Cdk5-/- mice. DiI crystals were placed in the cerebral
cortex of fixed brains of (A) P10 CxCdk5KO and (B) E18.5
Cdk5-/- mice and littermate controls. Vibratome sections
(100 µm) were imaged using a fluorescent (A,B) or confocal (inset in B)
microscope. (A) Abnormal dendrite development of commissural neurons in
CxCdk5KO mice. Scale bar: 25 µm. (B) In Cdk5+/+ mice,
cortical pyramidal neurons extended their apical dendrites toward the pia. By
contrast, Cdk5-/- neurons extended their dendrites in
multiple directions. Inset, confocal image of abnormal dendrite structures of
Cdk5-/- commissural neurons.