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Fig. 4. Development of adipocytes from the NC during mouse development.
Permanent genetic lineage labelling of pre- and post-migratory NC was achieved
by crossing transgenic mice carrying a Sox10-Cre construct into a
R26-YFP reporter. Double immunolabelling of P28
Sox10-Cre/R26-YFP offspring with anti-GFP (green) and anti-perilipin
(red) antibodies was used to identify NC derivatives and adipocytes,
respectively. Bisbenzimide was used to identify cell nuclei (blue). Sections
show salivary gland and ear (A-D), peri-ovarial (E-H) and trunk
subcutaneous (I-L) regions. There is almost complete colocalisation of
YFP and perilipin in the salivary gland area, whereas no overlapping can be
detected in the ovary and trunk subcutaneous adipose depots. Scale bar: 50
µm.
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