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Fig. 3. Overexpression of VEGF perturbs skeletogenesis. Sea urchin
embryos overexpressing VEGF following microinjection of VEGF
mRNA into the egg. (A) WMISH with a Ske-T probe at the
gastrula stage. Vegetal view. (B-D) DIC images of pluteus-stage
embryos. Arrow, supernumerary elements; circle, abnormal branching.
(E-J) WMISH with probes for (E,H) MSP130 at prism stage, (F,I)
SM30 and (G,J) MSP130 at pluteus stage.