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Fig. 6. Rescue of spicule formation by expression of VEGF mRNA in sea
urchin embryos injected with Mo-VEGF. (A) Co-injection of Mo-VEGF
and VEGF mRNA into unfertilized eggs. (B-D) Phenotypes of
72-hour-old embryos treated as in A. (E) Localized ectopic expression
of VEGF obtained by microinjection of Mo-VEGF into the egg, and
VEGF mRNA together with a fluorescent lineage marker into one
blastomere at the 8-cell stage. (F-K) 72-hour-old embryos treated as in
E were split into two batches for (F-H) DIC and fluorescence microscopy, with
the DIC/fluorescence signal superimposed to show spicule formation, or (I-K)
immunolocalization of lineage marker (light red) and WMISH with a
SM30 probe (brown); dispersed spherical red `dots' are microinjection
artifacts.
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