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Figure 1


Fig. 1. Skeletal abnormalities in Msx2-Cre; Bmpr1aflox/null limbs. (A) Mating scheme used to produce mutant (Msx2-Cre; Bmpr1aflox/null) and control (Bmpr1aflox/wt) mouse embryos. Inset, dorsal view of E10.5 normal (left) and mutant (right) forelimb buds stained via whole-mount ISH with a riboprobe specific for the Cre-deleted Bmpr1a exon. Note the lack of staining (arrowheads) in the mutant AER. (B,C) Lateral views of early mutant forelimb (B) and hindlimb (C) bud transgenic for the Cre reporter R26R and stained for ß-gal. Recombination (blue) in limb bud pre-AER (arrows) is extensive at the stages indicated. (D-I) Skeletal preparations of normal (D,F,H) and mutant (E,G,I) neonates. In mutants, the hindlimbs are absent (E) and the pelvic bones are reduced or absent (G). Mutant forelimbs contain all skeletal elements (E,I) with broader tips in distal phalanges (asterisks in I). AER, apical ectodermal ridge; IL, ilium; IS, ischium; PU, pubis; ss, somite stage.





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