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Figure 4


Fig. 4. AER-specific expression of Msx2 and Bambi are downregulated in mutant forelimb. Whole-mount ISH using probes specific for Msx2 (A,B) and Bambi (C-F) in normal (A,C,E) and mutant (B,D,F) mouse forelimb buds at the embryonic stages indicated. At E10.5, mutant Msx2 expression is absent (arrowheads in B), whereas mutant Bambi expression is reduced at E10.5 (arrowheads in D) and absent by E12.5 (arrowheads in F) specifically in the AER, although expression is extended in the subapical mesenchyme (F).





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