DEV02857 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Cardiomyocytes exhibit two morphologically distinct phases of cell movement during cardiac fusion. Time-lapse in a wild-type embryo expressing Tg(cmlc2:egfp), with time points 2 minutes apart. For analysis of cell movements, see Fig. 1. Lateral domains of egfp expression are not contiguous with the cardiomyocytes and represent ectopic transgene expression.

• Movie 2 -

  Movie 2. Transition to angular movement does not occur in the absence of endocardium. Time-lapse in a clo mutant embryo expressing Tg(cmlc2:egfp), with time points 3 minutes apart. For analysis of cell movements, see Fig. 2.

• Movie 3 -

  Movie 3. mil mutant cardiomyocytes exhibit angular movement despite the loss of initial medial movement. Time-lapse in a mil mutant embryo expressing Tg(cmlc2:egfp), with time points 2 minutes apart. For analysis of cell movements, see Fig. 3.

• Movie 4 -

  Movie 4. In mil;clo double mutants, cardiomyocytes exhibit no net directed movement. Time-lapse in a mil;clo mutant embryo expressing Tg(cmlc2:egfp), with time points 2 minutes apart. For analysis of cell movements, see Fig. 4.