|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. No angular movement in the absence of endocardium.
(A,B) Selected images from a time-lapse of cardiac fusion in a
typical clo mutant zebrafish embryo expressing
Tg(cmlc2:egfp) (see Movie 2 in the supplementary material),
exhibiting cardiac morphology at the (A) 17-somite and (B) 20-somite stages.
Dorsal views, anterior to the top. (C-E) Paths traveled during the
(C,D) entire 90 minutes and (E) final 20 minutes of fusion (arrows and
asterisks as described for Fig.
1). Tracks displayed represent a subset of the tracked cells in
this time-lapse. In clo mutants, cardiomyocytes exhibit medial
movement throughout the duration of cardiac fusion. Scale bar: in E, 20 µm
for A-E. (F) Radial bar graph (see
Fig. 1J,L) depicting degree of
displacement for all tracked clo mutant cardiomyocytes. Few cells
exhibit angular movement, resembling the pattern of cell behavior observed
during the first phase of wild-type fusion (see
Fig. 1J). (G,H)
Location of cardiomyocytes moving angularly in clo mutants. In
anterior (purple) and posterior (blue) regions (
), the
percentage of cells moving angularly is significantly reduced
(t-test, P<0.01) compared with that observed during the
second phase of wild-type fusion (see Fig.
1M). See Table 1
for additional data. (I,J) In situ hybridization depicts
cmlc2 expression in wild-type (I) and clo mutant (J) embryos
at 28 hpf. Dorsal views, anterior to the top; both images shown at the same
magnification. Elongation of the heart tube is delayed and aberrant in
clo mutant embryos.
|
