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Fig. 4. Endocardium directs cardiomyocyte movement, even in the absence of
initial medial movement. (A-D) Selected images from a time-lapse of
cardiac fusion in a mil;clo double-mutant zebrafish embryo expressing
Tg(cmlc2:egfp) (see Movie 4 in the supplementary material),
exhibiting cardiac morphology at the (A) 19-somite and (B) 23-somite stages.
Dorsal views, anterior to the top. (C,D) Paths traveled by cardiomyocytes in
the right lateral heart field during the entire time-lapse (asterisks as
described in Fig. 1). Images in
C,D are three times the magnification of those in A,B. Scale bar: 20 µm.
Example shown is representative of four heart fields analyzed. Cardiomyocytes
in mil;clo double mutants exhibit no net directed movement.
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