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Figure 6


Fig. 6. Zfrp8 and centrosome organization. Confocal projection through one layer of lymph gland cells from squashed (A,B) and intact (C-F) lymph glands of wild-type (A,C,E) and Zfrp8 mutant (B,D,F) Drosophila. (Df(2R)SM206 and Zfrp8M-1-1 give similar results.) Cells were stained with anti-{gamma}-Tubulin (A-E), anti-Dgrip91 (C,D) and anti-Dgrip84 (F) to visualize centrosomes. Confocal sections of lymph glands (E,F) show Zfrp8-HA adjacent to centrosmes (arrows). DNA is stained with Hoechst 33258. Scale bar: 5 µm.





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