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Fig. 7. Inactivation of one copy of the Bmp4 gene in
a Grem1-deficient mouse embryo results in formation of
two normal kidneys. (A-C) Whole-mount in situ hybridisation to
detect Pax2 expression at E14.5. (A) Wild-type Pax2
distribution. (B) The absence of Pax2 expression in a
Grem1-deficient embryo is due to the complete renal aplasia at this
stage. (C) The Pax2 distribution in kidneys of a
Grem1
/; Bmp4/+
embryo is indistinguishable from a wild-type littermate (A). (D) Gross
morphology of the wild-type urogenital system at birth (postnatal day P0).
(E) Haematoxylin and Eosin staining of a histological section of a
wild-type kidney 30 days after birth (P30). (F) PAS staining reveals
the morphology of the medulla (collecting ducts) and cortex regions of a
wild-type kidney at about 8 months of age (P250). (G) Gross morphology
of the urogenital system of a Grem1/;
Bmp4/+ mouse at birth (P0). Note that both kidneys
are of normal size and shape (compare with D). (H) Haematoxylin and
Eosin staining of a kidney section from a
Grem1/; Bmp4/+
mouse at P30. (I) PAS staining of a kidney from a
Grem1/; Bmp4/+
mouse at P250. Medulla, cortex and glomeruli appear normal. Bl, bladder; cd,
collecting duct; cx, cortex; gl, glomerulus; go, gonad; ki, kidney; md,
medulla, tu, tubules.
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