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Fig. 1. Cell death in Drosophila embryos that lack
dPSR. Embryos from flies containing previously identified
genomic deletions were screened by Acridine Orange at 8-10 hours of
development. (A) In yw control embryos, apoptotic cells were
observed to cluster together because of their engulfment by hemocytes. The
inset shows a higher magnification image to illustrate clustering. (B)
A lack of apoptotic cell clustering was observed in embryos homozygous for
Df(3R)5C1, ry506. (C)
dPSR null embryos have clustered AO-stained cells. (D) The
genomic region of dPSR is shown. The gray arrowhead indicates the
location of the P-element insertion. The dashed lines indicate the region
deleted in dPSRFM1. The translation start and stop sites
are indicated. The Jmjc domain and the putative transmembrane domain are
indicated in green and orange, respectively. Primers PSR1 and PSR12 were used
for inverse PCR and sequencing of the breakpoint. The remaining dPSR sequence
does contain an ATG; however, it is located at the 3' end of the JmjC
homology and putative transmembrane regions.
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