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Fig. 3. dPSR is localized in the nucleus of the cell in
Drosophila. (A) Expression of ECFP in S2 cells. ECFP
fills the cytoplasm of the cell. (B) dPSR was fused to the N-terminal
of ECFP and expressed in S2 cells. (C) PSR:ECFP cells stained with
Hoechst 33342 and (D) the overlay of PSR-ECFP and Hoechst 33342
visualized by fluorescence microscopy. The yellow arrows indicate identical
cells in each of panels B,C,D. PSR-ECFP co-localizes with the DNA dye Hoechst
33342.